With paying attention to factors such as yield of purification and concentration of separated protein by each of magnetic nanocomposite, it could be concluded that the length of linkers played an important role in α-amylase protein separation. To investigate the purifying application, sodium dodecyl sulfate polyacrylamide gel electrophoresis, one-dimensional isoelectric focusing gel electrophoresis, and alpha-amylase activity assay were employed. All of the magnetic nanocomposites were characterized and investigated by using Fourier-transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, field-emission scanning microscope, X-ray diffraction pattern, and vibrating-sample magnetometer analyses. The mentioned magnetic nanocomposites were applied to separate α-amylase protein from fresh bovine milk.

Three modified magnetic nanocomposites by dopamine were offered with different type of linkers.

In this paper, a novel nanocomposite based on magnetic nanoparticles decorated by dopamine were reported.

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